Acceptance rate | 46% |
---|---|
Time to first decision | 6 months* |
Time to decision with review | 50 days* |
*Approximate number of days
**The days mentioned above are averages and do not indicate exact durations. The process may vary for each article.
ACTA Pharmaceutica Sciencia
2005 , Vol 47 , Num 3
ROLE OF SOME FERMENTATION PARAMETERS AFFECTING LIPASE PRODUCTION BY FUSARIUM SOLANI
Natural and Microbial Products Chemistry Department, National Research Center (NRC), Ddokki, Cairo, Egypt
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The lipase production by F. Solani was inwestigated on using shaking flask and laboratory stirred fermenter. The results indicated that the best yield 380 U/ml (specific enzyme activity 9.04 U/mg protein) was obtained by using Erlenmeyer flask 250 ml containing 50 ml of culture with a medium composition g%: Sucrose 2, corn oil 0.2 ml, MgSO4.7H2O 0.05, KCI 0.05, KH2PO4 0.1, (NH4)2SO4 0.23, at pH 6.2 using citrate phosphate buffer and inoculum size 6 ml/50 ml medium. However, on using a bench top fermenter 5 L, the yield was increased by about one and half fold (520U/ml) under optimized fermentation conditions.
Keywords :
LIPASE PRODUCTION, F SOLANI