ACTA Pharmaceutica Sciencia Accepted Manuscripts
Anticancer potential of Pistacia vera L. pollen shell in-vitro using human osteosarcoma cell line MG63
Solmaz ZAKHIREH 1-2 Yadollah OMIDI 3 Younes BEYGI-KHOSROWSHAHI 4 Elham MOHAJEL KAZEMI 5 Jaleh BARAR 6-7 Khosro ADIBKIA 1-6-7
1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, 7 FL 33328, USA
4 Chemical Engineering Department, Faculty of Engineering, Azarbaijan Shahid Madani University, Tabriz, Iran
5 Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
6 Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
7 Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Pollen shells (PShs) are natural polymeric microcapsules that can be used as medical devices such as drug carriers. In the present study, for the first time, the anticancer potential of Pistacia vera L. PSh was investigated in-vitro using cell line MG63. Optical microscopy was used to study the PSh-cell interface. MTT assay was performed to assess cell cytotoxicity on the PSh microcapsules. The apoptotic activity of PShs was examined using the qRT-PCR technique. DAPI staining was benefited to visualize nuclear morphological changes in cells cultured on the PShs. Optical imaging indicated a bioactive PSh-cell interface and MTT assay results showed an anti-proliferative effect of PShs on the MG63 cells. Moreover, from the expression analysis of apoptosis-related genes (BAX and BCL2) and DAPI staining, PShs was found to be an apoptosis-inducing delivery vehicle against MG63 cells. Hence, such a microcapsule is proposed to be used as a drug carrier with anti-bone tumor activity. Keywords : Pollen, Pistacia vera L., Anticancer, Bone-targeted drug delivery

Istanbul Medipol University