ACTA Pharmaceutica Sciencia Accepted Manuscripts
Total phenolic contents, in vitro antioxidant activity, enzymes inhibition and anti-inflammatory effect of the selective extracts from the Algerian Lavandula multifida
Aissa MAMMERI 1-2 Hamdi BENDIF 3-4 Chawki BENSOUICI 5 Abderrahim BENSLAMA 6 Khellaf REBAS 3 Amina BOUASLA 7 Imane REBAIA 7 Nabila SOUILAH 8 Mohamed Djamel MIARA 9
1 Department of Chemistry, Faculty of Sciences, University of Msila, Msila, Algeria
2 Laboratory of Inorganic Materials, Faculty of Sciences, University of Msila, Msila, Algeria
3 Department of Natural and Life Sciences, Faculty of Sciences, University of Msila, Msila, Algeria
4 Laboratory of Ethnobotany and Natural Substances, Department of Natural Sciences, ENS Kouba, Algiers, Algeria
5 Biotechnology Research Center, UV 03, BP E7, Ali Mendjeli, Constantine. Algeria
6 Department of Microbiology and Biochemistry, Faculty of Sciences, University of Msila, Msila, Algeria
7 National Higher School of Biotechnology Taoufik Khaznadar, Constantine, Algeria
8 Department of Natural and life Sciences, Faculty of Sciences, University of Skikda, Algeria
9 Department of Nature and Life Sciences, Faculty of Nature and Life Sciences, University Ibn Khaldoun, Tiaret, Algeria
The present research aimed to carry out the phytochemical analysis, in vitro antioxidant activity, enzymes inhibition and anti-inflammatory effect of the selective extracts of the Algerian L. multifida from two regions; Msila region and Constantine region. The total phenolic and flavonoids contents of crude extract (CE) and its solvent partition fractions: dichloromethane (DME), ethyl acetate (EAE) and n-butanol (BUE) was determined spectrophotometriclly. The antioxidant activity of extracts was achieved by the use of seven methods and the enzyme inhibitory activity of extracts was evaluated against ?-amylase and butyrylcholinesterase. Moreover, the in vivo anti-inflammatory activity of the aqueous extract of L. multifida from Constantine region was evaluated using paw edema model. The M.EAE present the highest TPC and TFC, which were about 462.23±11.74?g GAE/mg extract and 125.90±0.16?g QE/mg extract, respectively. In addition, the M.EAE exhibited an excellent antioxidant activity, as it has a great ability to scavenging the DPPH, ABTS and galvinoxyl free radicals, as well as reducing power and metal chelating. However, the M.CE and C.CE showed the best inhibitory activity of the ?-amylase butyrylcholinesterase (IC50=64.17±1.81?g/ml and 83.55±1.97?g/ml, respectively. The preliminary investigation reveals that the EAE has a good biopharmaclogical activity, which it possess an interesting potential for pharmaceutical/nutraceutical applications. Keywords : Antioxidant activity, Anti-inflammatory effect, Enzymes inhibition, Lavandula

Istanbul Medipol University