ACTA Pharmaceutica Sciencia 2019 , Vol 57 , Num 2
Anti-inflammatory and Analgesic Potential of Acetone Leaf Extract of Combretum Sordidum and its Fractions
Babatunde B. Samuel Olayinka A. Oridupa 2 Fisayo Gbadegesin 1
1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan
2 Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ibadan
DOI : 10.23893/1307-2080.APS.05709 Combretum species are traditionally used in treatment of arthritis, fungal and bacterial infections. This study investigated anti-inflammatory activity of C. sordidum acetone extract in egg albumen-induced paw oedema and formalin paw lick test in rats, while analgesic activity was determined by acetic acid-induced abdominal writhing test in mouse. Bioactivity guided analgesic effect were also carried out on solvent-solvent partitioned and chromatographic fractions. Rats administered with extract progressively showed significant reduction in oedema formation 60 minutes post-induction. Dose-dependent inhibition of formalin-induced paw lick was observed at early and late phases of the experiment compared to indomethacin. Abdominal writhing was significantly inhibited with 400mg/kg extract effect (73.1%) comparable to aspirin (75.9%). Most active chromatographic fraction identified as F7 (79.5%) showed significant analgesic activity, higher than aspirin (55.0%). Results established anti-inflammatory and analgesic of C. sordidum. Further studies are on-going to identify and characterize the bioactive principle responsible for these pharmacological effects. Keywords : Combretum sordidum, Anti-inflammatory, Analgesic, Bioactivity-guided separation

Istanbul Medipol University