ACTA Pharmaceutica Sciencia 2010 , Vol 52 , Num 4
EFFECT OF KETOCONAZOLE AND JAVA PLUM PRETREATMENT ON INTESTINAL TRANSPORT OF BUSPIRONE ACROSS RAT INTESTINE
SHRAVAN KUMAR YAMSANİ, RAMESH GANNU, ADUKONDALU DEVANDLA, VAMSHİ VİSHNU YAMSANİ, CHİNNA REDDY PALEM, SHİVA KUMAR RAVULA, MADHUSUDAN RAO YAMSANİ, SARANGAPANİ MANDA
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, A.P. India The effect of ketoconazole and java plum on intestinal transport of buspirone, a CYP3A4 substrate across the rat intestine was investigated using normal sac technique. Three groups of rats, one serves as control; treated with ketoconazole and java plum juice for 7 days respectively in second and third group. The rats were sacrificed and intestinal segments (duodenum, jejunum and ileum) and colon were isolated and used for the studies. The buspirone solution was placed in the isolated intestinal sac. Samples were collected at preset time points and buspirone transported was estimated. The transport of buspirone was increased (p<0.05) 12.5, 5, 5.2 and 5.3 times after pretreatment with ketoconazole; 19.5, 8.5, 10.7 and 7.2 times after pretreatment with java plum juice compared to control. It suggests that both ketoconazole and java plum juice might be acting by inhibiting the transporters and enzymes which are responsible for transport/metabolism of buspirone. Keywords : KETOCONAZOLE, JAVA PLUM, BUSPIRONE, NORMAL SAC, INTESTINAL TRANSPORT

Istanbul Medipol University