| Submission to first decision
(The average time from manuscript submission to desk rejection, direct rejection/acceptance or assignment to the first editor) Note: For manuscripts that fully comply with the journal template. However, this timeframe does not apply to manuscripts with formatting or guideline deficiencies. If a manuscript is returned to the authors for corrections, the administrative review process will restart from the re-submission date. |
3 weeks |
|---|---|
| Submission to decision after review (For papers that are sent to review, the average time from submission to receipt of first editor decision) | 6 weeks |
| Submission to final decision (The average time from manuscript submission to the final editorial decision) | 16 weeks |
| Submission to publication (The average time from acceptance to publication. Manuscript published in early view are also included in this calculation) | 24 weeks |
ACTA Pharmaceutica Sciencia
2023 , Vol 61 , Num 3
Development of a liquid chromatographic method to monitorization of medazepam and lorazepam in plasma and its validation
1 Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Cumhuriyet University, 58140 Sivas, TürkiyeDOI : 10.23893/1307-2080.APS6116 Viewed : 9607 - Downloaded : 3416 This study, it was aimed to develop a simple, sensitive and reliable high-performance liquid chromatographic method for simultaneous analysis of medazepam and lorazepam based on the solid-phase extraction from human blood. For the pretreatment of (500 µL) plasma sample, an efficient extraction method was developed and optimized. Separation was carried out with an ODS reverse phase C18 analytical column (150x4.0mm, 3µm). The composition of 20 mM KH2PO4 buffer and methyl cyanide (6:4, v/v) was employed as the mobile phase in the chromatographic system. The ultraviolet detector was set at 220nm. Determination of coefficients values was found as 0.9928 (r2) between 500-2500 ng/mL concentrations for medazepam and 0.9983 between 20-300 ng/mL for lorazepam. It was observed that the method has successful validation test results from accuracy, sensitivity, recovery, precision, and robustness in accordance with ICH Q2R1 guidelines. The method is recommended for monitoring blood levels of lorazepam and medazepam in toxicology laboratories. Keywords : Medazepam, lorazepam, solid-phase extraction, HPLC-UV, method validation