ACTA Pharmaceutica Sciencia Accepted Manuscripts
Application of near-infrared spectroscopy and multivariate methods for the estimation of isopropyl alcohol content in hand sanitizer formulation
Sathish DHARANI 1 Tahir KHUROO 1 Sogra FB ALI 1
1 Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USA
To address the need of alcohol-based hand sanitizers during COVID-19, US FDA has issued a guidance for the preparation of hand sanitizers that recommends 80% v/v ethanol or 75%v/v isopropyl alcohol (IPA) along with other ingredients. The aim of this study was to develop a new method to estimate IPA content in hand sanitizers by using Near-infrared (NIR) spectroscopy with a multivariate chemometric approach. Calibration samples containing 10-90% of IPA were used for model development. NIR data were mathematically pretreated with multiple scattering correction before development of partial least squares (PLSR) and principal component regressions (PCR) model. Both models showed good linearity over the selected range of IPA content with high R2 (>0.993), low rootbroad mean squared error (<2.163), minimum difference between standard errors between calibration and validation models (0.0009). The proposed NIR technique with multivariate approach provides rapid analysis of IPA content in the hand sanitizer. Keywords : Isopropyl alcohol, disinfectant, near infrared, partial least square, calibration validation

Istanbul Medipol University