ACTA Pharmaceutica Sciencia 2010 , Vol 52 , Num 2
IDENTIFICATION AND DETERMINATION OF L-GLUTAMIC ACID AND L-ARGININE BY HPLC IN FOOD ADDITIVES
D TSVETKOVA, IV PENCHEVA, D OBRESHKOVA
Medical University - Sofia, Faculty of Pharmacy Department of Pharmaceutical Chemistry 2 Dunav str., 1000 Sofia, Bulgaria New analytical method for quality control of L - Glutamic acid and L - Arginine in food additives by terms of HPLC is developed and validated in respect of analytical parameters: selectivity, precision, accuracy, linearity, limit of detection = 4.0 μg/ml, limit of quantitation = 40.0 μg/ml. Precision is estimated by SD and RSD (%): SD = 0.02, RSD = 4.76 % (L - Glutamic acid in Tonotyl solution); SD = 0.05, RSD = 4.85 % (L - Arginine in Sargenor amp.). Accuracy for L - Glutamic acid is represented by the degree of recovery - R (%): 99.19 % 102.08 % and RSD (%): 1.90 % 5.69 %. Keywords : L - GLUTAMIC ACID, L - ARGININE, HPLC, FOOD ADDITIVES

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