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ACTA Pharmaceutica Sciencia
2005 , Vol 47 , Num 1
STUDY OF ENZYME ACTIVITY FOR PEPSIN AND ITS COMPLEXATION WITH DEXAMETHASONE AND THEIR CHARACTERIZATION
Faculty of Pharmacy, Pharmaceutical and Phyto Chemistry Group, University Of The Punjab Allama Iqbal Campus, Lahore-Pakistan
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The enzyme activities for pepsin and its complexation with dexamethasone were carried out. The pepsin activity both in free and complex form was studied by spectro fluorimeter using a newy prepared fluorosubtrate and proved to show the enzyme activity. The immunoreactivity of dexamethasone-Pepsin complex was also studied by rapid enzyme linked immunosorbent (ELISA) assay which proved that this complex was immunoreactive to dexamethasone specific antibodies. These studies will be useful for the preparation of a variety of drug-enzyme complexes.
Keywords :
PEPSIN, DEXAMETHASONE, COMPLEXES, ELISA