| Submission to first decision
(The average time from manuscript submission to desk rejection, direct rejection/acceptance or assignment to the first editor) Note: For manuscripts that fully comply with the journal template. However, this timeframe does not apply to manuscripts with formatting or guideline deficiencies. If a manuscript is returned to the authors for corrections, the administrative review process will restart from the re-submission date. |
3 weeks |
|---|---|
| Submission to decision after review (For papers that are sent to review, the average time from submission to receipt of first editor decision) | 6 weeks |
| Submission to final decision (The average time from manuscript submission to the final editorial decision) | 16 weeks |
| Submission to publication (The average time from acceptance to publication. Manuscript published in early view are also included in this calculation) | 24 weeks |
ACTA Pharmaceutica Sciencia
2019 , Vol 57 , Num 1
Development and Validation of UPLC Method for the Determination of Olopatadine Hydrochloride in Polymeric Nanoparticles
1 Cukurova University, Faculty of Pharmacy, Pharmaceutical Technology, Adana, Turkey2 Anadolu University, Faculty of Pharmacy, Pharmaceutical Technology, Eskişehir, Turkey
DOI : 10.23893/1307-2080.APS.05701 Viewed : 19509 - Downloaded : 6572 A new, simple, rapid, precise, accurate and specific stability indicating reverse phase UPLC method developed for the determination of encapsulated olopatadine hydrochloride (OLO) in the polymeric nanoparticle formulations. Studies were carried out on a 2.1x50 mm, 1.8 ?m Zorbax Eclipse Plus C18 column with an optimized mobile phase of methanol, water and sodium acetate buffer solution (40:50:10, v/v/v) at a flow rate of 0.5 mL/min. OLO was detected and quantitated using a photodiode array detector at a wavelength of 246 nm and the column temperature was adjusted to 40 °C. The proposed method validation was carried out for specificity, linearity, accuracy, precision, limit of detection, limit of quantitation and robustness according to the ICH harmonised tripartite guideline "validation of analytical procedures Q2(R1)". Analytical curve was linear over the concentration range of 5-50 ?g/mL. All the validation parameters were within the acceptance range. LOD and LOQ for OLO were 0.7652 and 2.3188 ?g/mL, respectively. The developed method fulfilled the requirements for reliability and feasibility for the quantitative analysis of OLO in polymeric nanoparticles. Keywords : Olopatadine hydrocloride, Eudragit, UPLC, validation, nanoparticles