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3 weeks |
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| Submission to final decision (The average time from manuscript submission to the final editorial decision) | 16 weeks |
| Submission to publication (The average time from acceptance to publication. Manuscript published in early view are also included in this calculation) | 24 weeks |
ACTA Pharmaceutica Sciencia
2023 , Vol 61 , Num 1
The optimal method to measure polyamines in serum by using HPLC fluorescence detector
1 Institute for Pharmacology and Toxicology, Giessen University, Germany2 Medical Laboratory Techniques, Vocational School of Health Services, Istanbul Medipol University, İstanbul, Türkiye
3 Department of Basic Medical Sciences, Department of Medical Biochemistry, Faculty of Medicine, Istanbul Medipol University, İstanbul, Türkiye
DOI : 10.23893/1307-2080.APS6107 Viewed : 8655 - Downloaded : 3004 Measurement of polyamines (putrescine, spermidine and spermine) is important in the monitoring of many metabolic events, as well as in cancer prognosis. However, the lack of method standardization and validation for the measurement of polyamines is a major obstacle. In this study, sample preparation, gradient program, chromatography column, sample concentration studies were attempted and an analysis method was developed for the detection of polyamines in serum by using HPLC fluorescence detector in order to reduce the difficulties experienced with the existing methodologies. This method was validated analytically in accordance with EMA and FDA"s guidelines. In this study, Waters Nova-Pak C18 A chromatography column with 3.9 mm, 150 mm and 4 µm specifications was used for the first time. Acetonitrile was used instead of methanol. Thanks to the newly developed gradient, peak purity and sufficient peak separation were ensured and accurate, sensitive, reliable and reproducible method was developed and validated. Keywords : Polyamines, HPLC, derivatization, O-Phthalaldehyde, N-Acetyl-L cysteine