Acceptance rate 46%
Time to first decision 20 days*
Time to decision with review 50 days*

*Approximate number of days

**The days mentioned above are averages and do not indicate exact durations. The process may vary for each article.


ACTA Pharmaceutica Sciencia 2010 , Vol 52 , Num 3
BIOANALYTICAL METHOD DEVELOPMENT AND ITS VALIDATION FOR DETERMINATION OF CANDESARTAN CILEXETIL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH UV DETECTION
ASHOK K PEEPLİWAL, CHANDRAKANT G BONDE, KRİSHNAPRİYA MOHANRAJ
School of Pharmacy and Technology Management, SVKM’s NMIMS University, Shirpur Campus Near Agra-Mumbai Highway, Shirpur, Dhule, Maharashtra, 425 405, India A simple, sensitive, fast method using isocratic high-performance liquid chromatography (HPLC) and ultra violet detection for the measurement of candesartan in human plasma was developed and validated. The HPLC column, a Phenomenex Luna C8, 250x4.6 mm, 56, was equilibrated with an eluent mixture of methanol - 10mM potassium dihydrogen phosphate (pH 3.0) with composition of (85:15 v:v) at 1 mL/min. Each analyte required no longer than 8 minutes. The peaks were eluted at 260 nm wavelength and no interferences found from plasma. Plasma samples were processed using acetonitrile as precipitating agent to extract candesartan. Quantitation was achieved by the measurement of peak area ratio and the absolute recovery varied from 96.92 to 101.07 %. Detection limit for candesartan in plasma was 20 ng/mL. Intra-day coefficients of variation (% CV) ranged from 0.55 to 2.41 and Inter-day (% CV) from 0.94 to 2.45 at three different concentrations. Keywords : BIOANALYTICAL METHOD, CANDESARTAN CILEXETIL, IRBESARTAN, UV-DETECTION

Istanbul Medipol University