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ACTA Pharmaceutica Sciencia
2026 , Vol 64 , Num 2
Correlation of interleukin-10, interleukin-17 and micro-RNA-938 expression profiles in some Iraqi women with toxoplasmosis
1 University of Baghdad, College of Science for Women, Department of Biology, IraqDOI : 10.23893/1307-2080.APS64011 Viewed : 218 - Downloaded : 86 Toxoplasmosis is an infectious parasitic illness resulting from Toxoplasma gondii, which involves intricate crosstalk with host immune pathways. Pathogenic progression includes both innate and adaptive immune mechanisms. miRNAs are an epigenetic factor crucial for regulating immune activity by interfering with cytokine signaling pathways. This study was conducted to determine the relationship between Anti-inflammatory cytokines (interleukin-10) and Proinflammatory cytokines (interleukin-17) in chronic toxoplasmosis cases. Serum samples were obtained from a total of 200 women, comprising 100 controls and 100 toxoplasmosis cases, aged 18?50 years, collected between November 2023 and September 2024. Before use, every sample was kept. For molecular study, miRNA-938 was measured in 100 whole blood samples, 50 patient samples, and 50 control samples utilizing Quantitative Real-Time PCR. Statistically, the findings exhibit no meaningful differences in microRNA-938 expression among the patient group (1.2368 ± 0.65981 ng/L) and the reference (healthy) group (1.239 ± 0.792 ng/L). The serum IL-10 levels in the patient group decreased by (13.658 ± 2.115 ng/L), more than those in the control (33.404 ± 5.973 ng/L). Whereas patients appeared elevated serum IL-17 levels (609.639 ± 45.184 ng/L) compared to control (207.778 ± 4.794 ng/L). Generally, our investigation revealed that the miR-938 expression remains unchanged during T. gondii infection, therefore, we suggest that miRNA-938 does not participate in changing immunological pathways, and the parasite has evolved strategies that have successfully manipulated host immunity. Additionally, dysregulation of the expression of both markers IL-10 and IL-17 gene could serve as an indicator for women infected with toxoplasmosis. Keywords : miRNA-938, cytokines, interleukins, toxoplasmosis, qRT-PCR