Acceptance rate 46%
Time to first decision 20 days*
Time to decision with review 50 days*

*Approximate number of days

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ACTA Pharmaceutica Sciencia 2022 , Vol 60 , Num 3
Efficacy of Liquid-Liquid Extraction and Protein Precipitation Methods in Serum Sample Preparation for Quantification of Fexofenadine in Human Serum
Farhana AFROZ 1-2 Fatema MONI 1 Suriya SHARMIN 1 Satyajit Roy RONY 1 Farhana AFROZ 1 Shah MASUM 2 Hossain SOHRAB 1
1 Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh
2 Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka-1205, Bangladesh
DOI : 10.23893/1307-2080.APS.6018 Biological samples contain many intrinsic and extrinsic compounds in different concentrations which makes it very challenging to analyze it and cannot normally be injected directly into the analyzing system without sample preparation. Two different sample extraction procedures liquid-liquid extraction and protein precipitation have been employed in this study to quantify fexofenadine in human serum and the recovery rates have been compared. Protein precipitation by methanol has an advantage over liquid-liquid extraction with recovery rates of more than 90% but has limitations due to rise of column back pressure. Due to better recovery rate and quick sample preparation technique the protein precipitation method has been chosen for extraction of drug from serum sample. The developed HPLC method was validated and found to be accurate, precise and specific within the linearity range of 0.8- 4.0 µg/mL. Lower limits of detection and quantification were established as 0.6 and 0.8 µg/mL respectively. Keywords : Liquid-liquid extraction, protein precipitation, bioanalysis, sensitivity

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